PDK4 polyclonal antibody
产品名称： PDK4 polyclonal antibody
英文名称： PDK4 polyclonal antibody
产品编号： PAB4565
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152
- 传真 : +886-2-8751-1186
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PDK4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human PDK4.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients.
Rosa G, Di Rocco P, Manco M, Greco AV, Castagneto M, Vidal H, Mingrone G.Obes Res. 2003 Feb;11(2):176-82.
- 2.
- Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading.
Razeghi P, Young ME, Ying J, Depre C, Uray IP, Kolesar J, Shipley GL, Moravec CS, Davies PJ, Frazier OH, Taegtmeyer H.Cardiology. 2002;97(4):203-9.
- 3.
- Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human.
Rowles J, Scherer SW, Xi T, Majer M, Nickle DC, Rommens JM, Popov KM, Harris RA, Riebow NL, Xia J, Tsui LC, Bogardus C, Prochazka M.J Biol Chem. 1996 Sep 13;271(37):22376-82.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PDK4 polyclonal antibody (Cat # PAB4565) in CEM cell line lysates (35 ug/lane). PDK4 (arrow) was detected using the purified PDK4 polyclonal antibody (Cat # PAB4565) (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 polyclonal antibody (Cat # PAB4565) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
- Entrez GeneID:
- 5166
- Protein Accession#:
- NP_002603;Q16654
- Gene Name:
- PDK4
- Gene Alias:
- FLJ40832
- Gene Description:
- pyruvate dehydrogenase kinase, isozyme 4
- Omim ID:
- 602527
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq
- Other Designations:
- pyruvate dehydrogenase kinase 4,pyruvate dehydrogenase kinase, isoenzyme 4
- Related Disease