HCMV (AD169 Strain) Purified Virus, β-PL Inactivated
产品名称： HCMV (AD169 Strain) Purified Virus, β-PL Inactivated
英文名称： HCMV (AD169 Strain) Purified Virus, β-PL Inactivated
产品编号： HCMV (AD169 Strain) Purified Virus, β-PL Inac
产品价格： 0
产品产地： 美国
品牌商标：
更新时间： 2023-09-19T20:51:30
使用范围： null
*Any orders of 5 or more for this item receive a 10% discount during checkout. Description（产品描述）：
Description(名称）
HCMV (AD169 Strain) Purified Virus, β-PL Inactivated
Catalog #（货号）
10-275-500
Size（规格）
0.5 mg
Price（价格）
请联系：0769-2289-0558， 0769-22890598， QQ:2533190771
Human Cytomegalovirus (AD169 strain) purified virus inactivated by beta propiolactone.
For research use only. Not for use in diagnostic procedures.
When research requires antigens that are no longer infectious, ABI offers select inactivated viruses. Methods of inactivation include: Detergent Treatment, UV-Inactivation, beta-Propiolactone, and Heat-Inactivation.
Depending on the application, the researcher must be careful in choosing which inactivation method is suitable for their needs.
Detergent: A chemical inactivation method that leaves no intact viral particles. The detergent dissociates most viral proteins. Suitable for ELISA, Western blot, and other protein based assays.
Ultraviolet: A physical and chemical inactivation method that leaves whole, intact viral particles. UV light crosslinks the product’s nucleic acid with minimal or no modification of the viral proteins. Suitable for ELISA, Western blot, and other protein based assays.
Beta-Propiolactone (ß-PL): A chemical inactivation method that modifies the nucleic acid and leaves whole, intact viral particles. ß-PL inactivation causes minimal or no modification of viral proteins. Suitable for ELISA, Western blot, and other protein based assays.
Heat: A physical inactivation method that leaves whole, intact virus, bacteria, or chlamydia elementary bodies. The heat inactivates the infectious agents, but leaves the nucleic acid unmodified. Suitable for nucleic acid based assays, such as standard and real time PCR.
Details（注意事项）:
Shipping and Storage: This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Recommendations: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendation: This inactivated biological preparation should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. 93-8395.
Product Disclaimer: β-Propropiolactone (β-PL) is an alkylating agent of nucleic acid that has been shown to be effective in the inactivation of viruses. β-PL chemically modifies viral nucleic acid without affecting the immunogenicity or protein structure; therefore, it is not appropriate for use in nucleic acid based-testing
Applications for use（适用范围）:
- ELISA
- Western blot
- Dot blot
- Other Protein-Based Assays
- Lymphocyte Stimulation Assay
- Antibody Screening/Titration
Quality control testing includes（质量控制检测包括）:
- Sterility
- Validation of Inactivation
- Protein Concentration
Certificate of Analysis（分析报告）:
Reference Articles（已发表参考文献）:
Engel P, Pérez-Carmona N, Albà MM, Robertson K, Ghazal P, Angulo A. “Human cytomegalovirus UL7, a homologue of the SLAM-family receptor CD229, impairs cytokine production.” Immunology and cell biology. 2011.
Ugarte-Torres A, Hoegh-Petersen M, Liu Y, et al. “Donor Serostatus Has an Impact on Cytomegalovirus-Specific Immunity, Cytomegaloviral Disease Incidence, and Survival in Seropositive Hematopoietic Cell Transplant Recipients.” Biology of Blood and Marrow Transplantation. 2010.
Brockman MA, Kwon DS, Tighe DP, et al. “IL-10 is up-regulated in multiple cell types during viremic HIV infection and reversibly inhibits virus-specific T cells.” Blood. 2009;114(2):346-356.
Burns CE, Galloway JL, Smith ACH, et al. “A genetic screen in zebrafish defines a hierarchical network of pathways required for hematopoietic stem cell emergence.” Blood. 2009;113(23):5776-5782.
Soeda A, Morita-Hoshi Y, Makiyama H, et al. “Regular Dose of Gemcitabine Induces an Increase in CD14+ Monocytes and CD11c+ Dendritic Cells in Patients with Advanced Pancreatic Cancer.” Japanese Journal of Clinical Oncology. 2009;39(12):797-806.
Morita-Hoshi Y, Heike Y, Kawakami M, et al. “Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation.” Bone Marrow Transplantation. 2008;41.
Huang KH, Loutfy MR, Tsoukas CM, Bernard NF. “Immune correlates of CD4 decline in HIV-infected patients experiencing virologic failure before undergoing treatment interruption.” BMC Infectious Diseases. 2008;8.
Rodriguez-Caballero A, Garcia-Montero AC, Almeida J, Balanzategui A, Munoz-Criado S, Orfao A. “Association between the HLA haplotype and the TCR-V? repertoire of anti-hCMV specific memory T-cells in immunocompetent healthy adults.” Cytometry Part B: Clinical Cytometry. 2007;72B(5):371-379.
Pasetti MF, Resendiz-Albor A, Ramirez K, et al. “Heterologous Prime–Boost Strategy to Immunize Very Young Infants against Measles: Pre-clinical Studies in Rhesus Macaques.” Clinical Pharmacology & Therapeutics. 2007;82(6):672-685.
Park K-D. “In vitro priming and expansion of cytomegalovirus-specific Th1 and Tc1 T cells from naive cord blood lymphocytes.” Blood. 2006;108(5):1770-1773.
Giese C, Demmler CD, Ammer R, et al. “A Human Lymph Node In Vitro–Challenges and Progress.” Artificial Organs. 2006;30(10):803-808.
Aandahl EM, Michaëlsson J, Moretto WJ, Hecht FM, Nixon DF. “Human CD4+ CD25+ Regulatory T Cells Control T-Cell Responses to Human Immunodeficiency Virus and Cytomegalovirus Antigens.” Journal of Virology. 2004;78(5).
Maecker HT, Ghanekar SA, Suni MA, He X-S, Picker LJ, and Maino VC. “Factors Affecting the Efficiency of CD8+ T Cell Cross-Priming with Exogenous Antigens.” The Journal of Immunology. 2001;166.