Product: Anti-MRP5 mAb, clone M5II-54
Cat. No.: MC-219 (1 mL)
MC-170 (0.5 mL)
Synonym:
ABCC5
Specificity:
M5II-54 reacts with an internal epitope of human
MRP5, a 190 - 200 kDa transmembrane protein
that is closely related to the multi-drug
resistance protein MRP. M5II-54 does not crossreact
with the human MDR1, MRP1, MRP2, or
MRP3 gene products.
Species Reactivity:
Human. Others not tested.
Ig Isotype:
Rat IgG2a
Immunogen:
A bacterial fusion protein containing amino acids
722-910 of human MRP5.
Hybridoma:
Lymph node cells from an immunized rat fused
with SP2/O mouse myeloma cells.
Format:
1 mL (> 200 tests) or 0.5 mL (> 100 tests) in
serum-free culture supernatant at a
concentration of 250 μg Ig/mL (concentration by
ELISA), with 0.1 sodium azide and protein
stabilizer. Sample has been filtered through a
0.22 μm filter.
Culture Medium:
IMDM supplemented with Nutridoma-NS/SP.
Medium does not contain serum or added
enzymes.
Storage:
Store at 4°C for short term. Aliquot and store at
-20°C for long term. Avoid repeated freeze/thaw
cycles.
Applications and Suggested Dilutions:

Flow Cytometry: Optimal conditions still to be
defined.

Immunocytochemistry: Use a 1:20 to 1:50
dilution on acetone-fixed cytospin
preparations.

Immunohistochemistry: Use a 1:20 dilution
on acetone-fixed frozen sections, followed by
incubation with rabbit anti-rat IgG and
monoclonal rat APAAP complex. Alternatively,
after incubation with primary antibody and
washing, slides can be incubated with
biotinylated rabbit anti-rat IgG and
streptavidin conjugated to horseradish
peroxidase.

Western blot: Use a 1:20 to 1:50 dilution (with
anti-rat-HRP).
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product